goat anti human kgf fgf7 antibody  (R&D Systems)

Journal:

Article Title: Interleukin-1? Is a Paracrine Inducer of FGF7, a Key Epithelial Growth Factor in Benign Prostatic Hyperplasia

doi:

Figure Lengend Snippet: Correlation of tissue concentration of IL-1α and FGF7 in hyperplastic prostate. Proteins were extracted from snap-frozen BPH tissue samples, and the concentration of Il-1α and FGF7 was determined by ELISA in each extract. A: Scatter diagram with best fit linear regression shown (r = 0.73). B: Data are expressed as the mean FGF7 concentration (± SEM) for BPH tissue in four groups: <200 pg/ml (n = 17); 200–400 pg/ml (n = 6); 400–600 pg/ml (n = 5); and >600 pg/ml (n = 6).

Article Snippet: After multiple washes with wash buffer, the plates were incubated with either biotinylated anti-human FGF7 (BAF251, R&D Systems) at 100 ng/ml or biotinylated anti-human IL-1α (BAF200, R&D Systems) at 50 ng/ml for 2 hours at room temperature.

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay

Journal:

Article Title: Interleukin-1? Is a Paracrine Inducer of FGF7, a Key Epithelial Growth Factor in Benign Prostatic Hyperplasia

doi:

Figure Lengend Snippet: Quantitation of the localization of FGF7-expressing cells in prostate tissue. Frozen sections of six normal peripheral zone and 11 BPH tissues were analyzed by immunohistochemistry with anti-FGF7 monoclonal antibody. The number of cells located adjacent to epithelial acini (periacinar) was compared to the number of cells in stromal areas, using a point counting protocol. The mean counts in the 10 fields (± SEM) in each location for the normal peripheral zone (PZ) and hyperplastic transition zone (BPH) is shown. For both types of tissue the difference between the periacinar and stromal tissues is statistically significant (P < 0.03, paired t-test).

Article Snippet: After multiple washes with wash buffer, the plates were incubated with either biotinylated anti-human FGF7 (BAF251, R&D Systems) at 100 ng/ml or biotinylated anti-human IL-1α (BAF200, R&D Systems) at 50 ng/ml for 2 hours at room temperature.

Techniques: Quantitation Assay, Expressing, Immunohistochemistry

Journal:

Article Title: Interleukin-1? Is a Paracrine Inducer of FGF7, a Key Epithelial Growth Factor in Benign Prostatic Hyperplasia

doi:

Figure Lengend Snippet: Stimulation of FGF7 secretion by epithelial conditioned medium. Primary cultures of prostatic stromal cells were plated in RPMI 1640 with insulin, transferrin, and selenium and supplemented with a 1:10 dilution of conditioned medium (CM) prepared from primary epithelial cultures or were not supplemented. At 24-hour interval aliquots of stromal media were removed and assayed in duplicate for FGF7 content by ELISA. Results with three different epithelial conditioned media are shown.

Article Snippet: After multiple washes with wash buffer, the plates were incubated with either biotinylated anti-human FGF7 (BAF251, R&D Systems) at 100 ng/ml or biotinylated anti-human IL-1α (BAF200, R&D Systems) at 50 ng/ml for 2 hours at room temperature.

Techniques: Enzyme-linked Immunosorbent Assay

Journal:

Article Title: Interleukin-1? Is a Paracrine Inducer of FGF7, a Key Epithelial Growth Factor in Benign Prostatic Hyperplasia

doi:

Figure Lengend Snippet: Inhibition of stimulation of FGF7 secretion by CM with inhibitors of IL-1 α action. A: Primary cultures of prostatic stromal cells were plated in RPMI 1640 with insulin, transferrin, and selenium and supplemented with a 1:10 dilution of conditioned medium (CM) prepared from two separate primary epithelial cultures (CM1 and CM2); diluted CMs preincubated with excess anti-Il-1α neutralizing antibody; diluted CM1 preincubated with anti-VEGF (vascular endothelial cell growth factor) antibody; Il-1α at 6 pg/ml; or Il-1α at 6 pg/ml preincubated with anti-Il-1α neutralizing antibody or were not supplemented (control). FGF7 concentration was determined by ELISA after 48 hours of treatment. B: Primary cultures of prostatic stromal cells were plated in RPMI 1640 with insulin, transferrin, and selenium and supplemented with a 1:10 dilution of conditioned medium (CM) prepared from two separate primary epithelial cultures (CM1 and CM2); diluted CMs after cells were pretreated with recombinant IL-1Ra or Il-1Ra only, or were not supplemented (control). FGF7 concentration was determined by ELISA after 48 hours of treatment.

Article Snippet: After multiple washes with wash buffer, the plates were incubated with either biotinylated anti-human FGF7 (BAF251, R&D Systems) at 100 ng/ml or biotinylated anti-human IL-1α (BAF200, R&D Systems) at 50 ng/ml for 2 hours at room temperature.

Techniques: Inhibition, Control, Concentration Assay, Enzyme-linked Immunosorbent Assay, Recombinant